Abstract:
Serotonin is of crucial importance for the animal body performing the control over important physiological functions, including ovulation, insulin secretion, as well as serotonin predetermines the specific behavioral reactions. Modern science has accumulated enough experimental material on the regulatory influence of this neurotransmitter on the activity
[...] Load More of immunocompetent organs. However, data on the participation of serotonin in their structural and functional development have not yet been properly considered. The purpose of the paper was to investigate the morphogen effects of serotonin synthesis intensity on thymus development and manifestations of immune system disorders among animals. The fetuses of 38 laboratory pregnant female Wistar rats and post-natal rats living under standard conditions were used as an experimental basis for the research. The object of the research was serotonin receptors encoded with the Htr1a gene as the most represented receptor in the animal body. Isoflurane inhalation anesthesia for animals was used to obtain embryonic material. The method of a polymerase chain reaction with reverse transcription was used to detect and research the dynamics of the expression level of Htr1а receptors to serotonin in the thymus of the animals under study. Observation and analysis of isolated total RNA were carried out with the help of electrophoresis in agarose gel and the ChemiDoc MP System for gel imaging and documentation. With the use of the western blotting analytical method, proteins have been detected and transferred to the membrane. The quality of the process was controlled by coloring the hydrophobic nitrocellulose membrane with the Ponceau S dye. The functional activity of T-lymphocytes of rat fetal thymus has been assessed through activation by T-cell mitogen to proliferative immune response. The degree of maturity of T-lymphocytes has been analyzed by double marking with monoclonal antibodies to CD8b and CD4 antigens in fetuses and mature mice. The effect of serotonin on T-lymphocytes and the effect of ConA mitogen on the expression of Htr1a mRNA receptors in the thymus have been analyzed using the polymerase chain reaction method. Analytical processing of the laboratory assessment data was performed using the Statistica 7.0 software product. The assessment of the statistical significance of differences between indicator sets was implemented based on the Mann-Whitney U test. The results of the study will be useful to find the possibility of correction of immune system disorders using serotonin receptor agonists and antagonists that change the mediator level in various organs, including the brain.
Keywords: mice, serotonin, synthesis, thymus, Htr1a gene, receptors, immune system.
Journal title: available for those who has paid.
Region of the journal publication: Asia.
Scientific field: Veterinary.
Indexing of the journal:Scopus (Q1-2); WoS (Science Citation Index Expanded).