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#25-med Type 1 diabetes mellitus and pregnancy: assessment of glycemic profile variability as the basis of insulin therapy strategy

Abstract: Currently, there is practically no evidence base confirming or refuting the effect of pathological glucose variability, the duration of hyperglycemic and hypoglycemic states on the frequency and severity of pregnancy complications in women with type 1 diabetes mellitus (DM). The research purpose is to develop criteria for assessing the state of carbohydrate [...] Load More metabolism (CM) in pregnant women with type 1 diabetes to determine the insulin therapy strategy and management tactics. The methodology used in the work is based on current approaches to the examination and treatment of patients with type 1 DM during pregnancy. The work was carried out according to the design of a comparative prospective study of the case-control type. The analytical method, empirical methods of observation, comparison, and logical analysis were used; methods for assessing the state of the CM were applied; an assessment of glucose variability, a study of indicators of the hemostasis system, an assessment of the frequency and severity of obstetric complications, as well as the condition of newborns from mothers with type 1 DM on a percentile scale were carried out; the results of using various regimens of insulin therapy for type 1 DM during pregnancy were analyzed; a prognostic model for the development of endothelial damage, the frequency and severity of obstetric complications in women with type 1 DM and the degree of perinatal complications were developed; indicators of the duration of hyperglycemia and the duration of the hypoglycemic state during the day, the degree and duration of glucose variability were studied; the relationship between glucose variability, the duration of hyperglycemic, hypoglycemic state, and endothelial damage, disorders in the hemostasis system in such women during pregnancy were demonstrated; the advantage of a regimen of continuous subcutaneous insulin infusion in achieving euglycemia during the day, reducing glucose variability, reducing the frequency and severity of hypoglycemic episodes, and reducing obstetric and perinatal complications in the examined women was confirmed; the prognostic significance of CM indicators was studied and confirmed; optimal management of pregnancy in women with type 1 DM was developed. Novelty of research: a comprehensive assessment of the parameters of CM in pregnant women with type 1 DM was carried out; the duration of hyperglycemia during the day, the duration of hypoglycemia, and the assessment of glucose variability using estimated coefficients were studied; for the first time, a comparative analysis of CM indicators was carried out in pregnant women with type 1 DM who used the regimen of continuous subcutaneous insulin infusion, and in pregnant women who received multiple insulin injections; the advantage of the regimen of continuous subcutaneous insulin infusion in achieving target glycemic values in pregnant women with type 1 DM, reducing the variability of glucose concentration and the frequency of hypoglycemic episodes when using various functions of the insulin pump was substantiated; it was proven that pathological glucose variability is a predictor of endothelial damage, it leads to disruption in the hemostasis system, reduces the functional state of the kidneys, and increases the frequency and severity of preeclampsia in pregnant women with type 1 DM; positive correlation dependence of pathological glucose variability and duration of hyperglycemic conditions in mother and neonatal complications in newborns from mothers with type 1 DM were revealed.
Keywords: pregnancy; glucose; insulin therapy; complications; type 1 diabetes mellitus; carbohydrate metabolism.
Date of publication: March 2024 / Deadline: 25 October 2023

Journal title: available for those who has paid.

Region of the journal publication: All countries.

Scientific field: Medicine, Endocrinology.

Indexing of the journal: Scopus Q 1+WoS (SCIE/SCI).

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#33 Obesity in working age men: hormonal-metabolic and hemodynamic risk factors for cardiac arrhythmias

Abstract: Obesity (Ob) contributes to the development of a number of cardiovascular risk factors associated with cardiac arrhythmias. Currently, the problem of the prevalence of heart rhythm disturbances and their nature, the relationship of arrhythmias with possible risk factors associated with Ob in patients most at risk of death from cardiovascular diseases [...] Load More – working age men (WAM) with Ob, has not been studied enough. The research purpose is to study the hormonal-metabolic and hemodynamic risk factors for cardiac arrhythmias in WAM with Ob. The design of this study is presented as a cross-sectional, comparative, non-randomized, and descriptive study of three groups of patients: with normal body weight (BW), overweight BW, and obese patients. In the work, clinical (including anthropometric parameters), laboratory (including hormonal blood test), instrumental (24-hour monitoring of the electrocardiogram (ECG)), 24-hour monitoring of blood pressure (BP, echocardiogram) research methods, testing of patients for probability of obstructive sleep apnea syndrome (OSAS) using the Berlin questionnaire and statistical research methods were applied. In the study, it was found that in order to identify the features of the heart rhythm and the presence of risk factors for arrhythmias during the examination of WAM with Ob, it is advisable to include Holter monitoring (HM), 24-hour blood pressure monitoring (24HBPM), assessment of the full lipid spectrum with a mandatory study of high density lipoprotein cholesterol (HDL cholesterol); it is necessary to study the level of adiponectin in men with Ob and the chronic heart failure (CHF) in determining the therapy techniques; if there is arrhythmia in patients with Ob under the age of 50, identified during a standard cardiac examination, including HM, additional studies are required to clarify the presence of coronary heart disease (CHD); a significant positive effect on the heart rhythm in patients with Ob is provided by the prescription of statins with the effects on lipid metabolism. The novelty of the research is: a detailed assessment of the structure of cardiac arrhythmia in WAM with different BW was carried out, a significantly higher frequency of arrhythmias, both supraventricular and ventricular, was established in the presence of Ob with a progressive increase in their frequency as its severity worsens; a significant relationship was evidenced between the incidence of atrial fibrillation and the degree of increase in BW; the association of dyslipidemia with atrial fibrillation and ventricular extrasystole was revealed; it was shown that a low level of HDL cholesterol increases the risk of atrial fibrillation by 3.77, ventricular extrasystole by 2.30 times; no significant effect of the level of glycemia, insulinemia, and insulin resistance on the heart rhythm was found; the possibility of an adverse effect of an increased content of adiponectin on the frequency of ventricular extrasystole was demonstrated; it was shown that the increase in the frequency of supraventricular extrasystole and ventricular extrasystole with age and Ob is due to the presence of CHD, in the absence of which this pattern was not observed; it was found that in patients with Ob, an increased risk of developing atrial fibrillation is due to such features as a change in the daily BP profile of the nondipper type and myocardial remodeling in terms of dilatation of the left ventricle and left atrium, the risk of ventricular extrasystole is due to a change in the daily BP profile of the nondipper type and left ventriclar hypertrophy and left ventriclar diastolic dysfunction, the risk of supraventricular extrasystole is due to the left ventriclar diastolic dysfunction; also a positive effect was shown in the treatment with rosuvastatin to reduce the daily amount of ventricular extrasystole in patients with Ob.
Keywords: obesity; cardiovascular risk; heart rhythm; working age.
Date of publication: March 2024 / Deadline: 25 October 2023

Journal title: available for those who has paid.

Region of the journal publication: Europe.

Scientific field: Medicine.

Indexing of the journal: Scopus (Q2); WoS (Science Citation Index Expanded ).

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#32 Metabolic, hormonal and genetic factors in the formation of the obesity phenotype, their significance in the development of kidney failure and the benefits of therapeutic medication

Abstract: The study of the obesity phenotype in working age people with the determination of genetic predictors and the identification of pathogenetic relationships between the components of the cardiorenal continuum with the assessment of the benefits of therapeutic medication in dynamics is an important area of scientific research that is of practical significance [...] Load More for modern health care. The research purpose is to study the input of the main pathogenetic factors in the formation of the obesity phenotype, to determine the relationship of hormonal-metabolic and genetic parameters with markers of renal dysfunction in groups, and to evaluate the effectiveness of therapeutic medication over time. When performing the work, clinical, laboratory, genetic, instrumental, and statistical research methods were applied. The study objects were patients with metabolically healthy obesity (MHO) and metabolically complicated obesity (CO) associated with arterial hypertension (AH), patients with AH without obesity; the referential group included healthy respondents without obesity. In the study, differences in obesity phenotypes were established in terms of parameters of the body composition; the gender feature of the development of leptin resistance was determined; a mathematical module to identify preclinical renal dysfunction in obese patients was developed; the optimal range of body weight loss (BW) from 5 to 15% of the baseline within 6 months, predictors of effective weight loss in patients with obesity and additional criteria for effectiveness were determined; a method for predicting the effectiveness of reducing body weight in obese patients by the index of free leptin was introduced into practice; prognostic markers for effective treatment of obesity in patients with metabolic disorders were developed. The scientific novelty is an expanded characterization of various obesity phenotypes based on complex laboratory and genetic studies; for the first time a comparative assessment of the state of the kidneys in patients with different obesity phenotypes was carried out; a mathematical module was developed to assess the formation of early renal dysfunction in patients with CO; predictors of an effective decrease in BW in patients with obesity were determined; prognostic markers of effective treatment of obesity in patients with metabolic disorders were proposed.
Keywords: metabolism; obesity; kidney failure; renal dysfunction; therapy.
Date of publication: March 2024 / Deadline: 25 October 2023

Journal title: available for those who has paid.

Region of the journal publication: Europe.

Scientific field: Medicine.

Indexing of the journal: Scopus (Q2), Web of Science (Science Citation Index Expanded, Q2) IF 3,5.

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#31 Dental implantation in patients with chronic obstructive pulmonary disease

Abstract: At present, with the expansion of indications for dental implantation, the problem of reducing the risk of complications, among the most significant causes of which is the general somatic pathology in the patient, becomes urgent. A significant number of somatic pathology, especially in patients over the age of 40 years old, is chronic obstructive pulmonary [...] Load More disease (COPD). At present, there is no systematic source of information on the dental status of COPD patients undergoing dental implantation. The research purpose is to increase the effectiveness of dental implantation in COPD patients. The study was carried out according to the principles and rules of evidence-based medicine. Clinical and functional methods (laser Doppler flowmetry, optical tissue oximetry), the method of resonant frequency analysis of implant stability, X-ray, and statistical research methods were applied. The object of the study were 43 patients diagnosed with partial loss of teeth and diagnosed with COPD. As a result of the studies on COPD patients after dental implantation, the features of the pathogenesis of osseointegration processes were revealed according to microhemodynamics and oxygenation data; at the stages of preparation and implementation of dental implantation, recommendations were made for antioxidant and anti-osteoporotic therapy in COPD patients; a plan for preoperative preparation and postoperative management of COPD patients during dental implantation was developed. Novelty of the research: for the first time, the clinical and functional features of the condition in the mucous membrane of the alveolar ridge in COPD patients were studied; for the first time a comparative analysis of laboratory data and data of ultrasonic densitometry and X-ray absorptiometry (axial skeleton) before and after osteotropic therapy in these patients was carried out; based on specific, osteotropic, and antioxidant therapy, normalization of mineral metabolism in bone tissue, good osseointegration of dental implants, and restoration of tissue blood flow in COPD patients were achieved.
Keywords: antioxidant therapy; dental implantation; osteotropic therapy; dentistry; chronic obstructive pulmonary disease.
Date of publication: March 2024 / Deadline: 25 October 2023

Journal title: available for those who has paid.

Region of the journal publication: Europe.

Scientific field: Dentistry.

Indexing of the journal: Scopus (Q2); WoS (Emerging Sources Citation Index).

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#17 Ovulatory function of the ovaries in women with type 1 diabetes mellitus in relation to the carbohydrate metabolism compensation

Abstract: The features of the reproductive system in women with type 1 diabetes mellitus, manifested in a high frequency of ovarian hormonal insufficiency, which is the leading cause of reproductive dysfunction, were studied. Despite the great attention of researchers to the issues of the pathogenesis of hormonal ovarian insufficiency in such women, this problem [...] Load More remains under-analyzed. In this regard, the research purpose is to study the violation of the ovulatory function of the ovaries and the possibility of its recovery in women of reproductive age with type 1 diabetes, depending on the carbohydrate metabolism compensation. In the study, the clinical and laboratory characteristics of the examined women were compiled based on: the results of clinical and laboratory, and hormonal examinations; determination of anti-ovarian antibodies in the blood in the blood serum of patients using enzyme immunoassay; assessment of the degree of compensation for type 1 diabetes by the level of glycemia and the content of glycated hemoglobin A1c in the blood; evaluation of the stage of diabetic nephropathy based on a general urinalysis, urinalysis for proteinuria, a test for microalbuminuria by quantitative and semi-quantitative methods, and functional tests; ophthalmological examination; ultrasound examination of the pelvic organs; statistical processing of the obtained results using the methods of parametric and non-parametric statistics; processing the material with the statistical tools of the Excel 2010 program, a standard software package for applied statistical analysis; methods of descriptive statistics; assessment of differences in the values of quantitative indicators in different groups according to Student’s t-test for independent samples and the parametric Mann-Whitney U-44 test; comparison of frequency and nominal values according to Pearson’s chi-squared test with Yates correction and Fisher’s exact test for small samples; correlation analysis based on the Spearman’s rank correlation coefficient (r). As a result of the study, it was found that 62.2% of women with type 1 diabetes of reproductive age have ovarian insufficiency; it was determined that the frequency of occurrence of ovarian insufficiency correlates with the compensation of metabolic disorders characteristic of diabetes, insulin dose and does not depend on the insulin regimen, and in patients with anovulatory cycles – a significant decrease in the level of FSH and globulin that binds sex steroids, as well as a significant increase in the level of total and free testosterone levels compared to healthy women. It was proved that ovarian insufficiency in women of reproductive age with type 1 diabetes mellitus is associated with the carbohydrate metabolism compensation, the insulin dose, an increase in the level of total and free testosterone, a decrease in sex steroid-binding globulin, an increase in volume and a change in the structure of the ovaries. The possibility of restoring the ovulatory function of the ovaries in 61.8% of women with decompensated diabetes with anovulation or luteal phase deficiency was confirmed when diabetes compensation was achieved. A number of practical recommendations was developed for wide use in the control of women with type 1 diabetes mellitus of reproductive age: to conduct hormone testing and ultrasound examination of the pelvic organs to assess the hormonal function of the ovaries; with ovarian insufficiency, to carry out more careful control of carbohydrate metabolism and optimize insulin therapy; to re-evaluate the hormonal function of the ovaries when compensation for diabetes in women of reproductive age is achieved; to settle the issue of the advisability of hormone replacement therapy for the prevention of menstrual irregularities and proliferative diseases of the reproductive system in women who do not plan pregnancy. The study is the first to demonstrate that the volume of the ovaries, the number of antral follicles in them in patients with type 1 diabetes mellitus are directly dependent on microvascular diabetic complications and the insulin dose. It was found that the level of progesterone in these patients with a preserved menstrual cycle has a significant negative correlation with the level of glycated hemoglobin. It was proved that the ovulatory function of the ovaries in women with decompensated diabetes and ovarian insufficiency can be restored in 61.8% of cases with compensation for type 1 diabetes. It was confirmed that the data on the normal level of anti-ovarian antibodies and the titer of antibodies to ovarian antigens in such women indicate the absence of the significance of autoimmune ovarian damage in the pathogenesis of ovarian failure in type 1 diabetes mellitus.
Keywords: gynaecology; type 1 diabetes mellitus; ovarian insufficiency; carbohydrate metabolism; ovaries.
Date of publication: March 2024 / Deadline: 25 October 2023

Journal title: available for those who has paid.

Region of the journal publication: Europe.

Scientific field: Medicine.

Indexing of the journal: Scopus (Q2); WoS (Science Citation Index Expanded) IF 3.5.

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#16 Autoantibody spectrum and blood cytokine profile in children with juvenile idiopathic arthritis and unspecified reactive arthropathy

Abstract: The research deals with the problem of increasing the number of children with chronic inflammatory joint disease, in particular with juvenile idiopathic arthritis (JIA), to diagnoze which the determination of antibodies against citrullinated peptide (ACP) is increasingly used. However, contradictory diagnostic data in children with such joint diseases [...] Load More determine the relevance of assessing the prevalence of autoantibodies (AAB), the level of cytokines (CK) in whole-blood cell culture supernatants. The research purpose is to evaluate the frequency of determination of AAB, the features of the production of pro- and anti-inflammatory CK in children with JIA and unspecified reactive arthropathy (nReA). The work was carried out according to the general scientific methodology based on a systematic approach applying formal-logical and specific methods. An integrated approach was used, including the analysis of outpatient records, laboratory and statistical methods. The frequency of occurrence of antinuclear antibodies (ANA) was studied; spontaneous and stimulated production of pro- and anti-inflammatory CK by peripheral blood cells, as well as the level of these proteins in blood serum, were evaluated. A comparative analysis of the obtained data with the results of the reference group was applied. The study found the frequency of antibodies against modified citrullinated vimentin (AMCV) and ANA in the reaction of indirect immunofluorescence in patients with JIA and with nReA; the most common types of substrate fluorescence. The research discovered that in patients with JIA there is an imbalance in spontaneous cytokine production, and in patients with nReA there is an increase in spontaneous production of both pro-inflammatory and anti-inflammatory CK by blood cells; when mitogen stimulated blood cells in children with JIA and nReA, there is an increase in TNF-α production, a decrease in the secretion of IL-1β, IL-8, IFN-γ, IL-1ra, compared to conditionally healthy children; in children with JIA, there are no differences between groups with positive and negative values of AMCV in clinical, radiological parameters and CK production, as well as between groups with positive and negative results of the determination of antinuclear factor (ANF). It is recommended to use the obtained data on AAT production and cytokine profile in chronic inflammatory joint diseases in children, rheumatology, and physiology. It is not required to determine the level of AMCV in nReA patients. It is proposed to simultaneously evaluate spontaneous and stimulated production of pro- and anti-inflammatory CK by blood cells in scientific and clinical practice. For the first time, a research of the frequency of detection of ANA and ACP in children with nReA was conducted; the production of pro- and anti-inflammatory CK by peripheral blood cells in patients with nReA was studied; a comprehensive study of spontaneous and stimulated synthesis of pro- and anti-inflammatory CK by peripheral blood cells in patients with JIA, nReA and in healthy children was carried out using a common methodological framework. The methodological approach with the use of mitogen stimulation made it possible to characterize the features of CK production by blood cells, which were not manifested in the study of their spontaneous synthesis. The methodological approach with the use of mitogen stimulation allowed characterizing the features of CK production by blood cells, which were not manifested in the study of their spontaneous synthesis.
Keywords: autoantibody; arthropathy; vimentin; peptides; cytokines
Date of publication: March 2024 / Deadline: 25 October 2023

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Region of the journal publication: Europe.

Scientific field: Medicine.

Indexing of the journal: Scopus (Q2); WoS (Science Citation Index Expanded; Social Sciences Citation Index ).

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#43-med Whole genome analysis of primary open-angle glaucoma associations with polymorphic loci of the LOXL1 gene

Abstract: Under modern conditions, primary open-angle glaucoma continues to occupy a leading place in the structure of visual disabilities, despite the success in early diagnosis, a wide range of conservative and surgical methods of treatment. The etiology of glaucoma has not been fully studied, because a multifactorial concept with the involvement of various [...] Load More genetic and non-genetic factors prevails in explanation of its origin and development. Therefore, the selection of polymorphic loci to find associations with the studied phenotype is important for a genetic and epidemiological survey. The purpose of this research was a whole genome analysis and substantiation of associations of primary open-angle glaucoma with polymorphic loci localized in the region 15q24.1. The 246 men with a clinical diagnosis of primary open-angle glaucoma made up an experimental sample of observations, 176 of which formed a control group. The control sample of the observed men has been formed on the principle of nationality homogeneity and lack of any kindred relationships among men. With the use of the phenol-chloroform extraction method, the genomic DNA has been isolated based on the generated panel of peripheral blood samples of the observed men. A sample of seven polymorphic gene loci was formed, whose reaction with the level of expression previously proven in other studies. For the purpose of the genotype comparative analysis of each of the observed patients on the basis of the method of the polymerase chain reaction of DNA synthesis, genotyping of the investigated polymorphic loci has been performed. Determination of the regulatory potential of polymorphisms using contingency tables with the phenotype under investigation has been performed on the platform of the HaploReg software package. The analysis and evaluation of the association in accordance with the Hardy-Weinberg law of genetic equilibrium have been performed using Pearson's chi-squared test. The method of multivariate logistic regression analysis was used in the framework of this research to study the relationship of reference and alternative alleles to the change in regulatory DNA motifs of recipients. The reflective research has confirmed the proactive value of polymorphic loci of the LOXL1 gene (GG rs893818 and GG rs4886776) in the development of primary open-angle glaucoma among men. The results of this research are valuable for understanding the specificity of particular genes in the development of visual defects across various ethno-territorial population groups and environmental conditions. Keywords: genotype, primary open-angle glaucoma, polymorphic loci, expression, whole genome analysis, LOXL1 gene.
Keywords: genotype, primary open-angle glaucoma, polymorphic loci, expression, whole genome analysis, LOXL1 gene.
Date of publication: March 2024 / Deadline: 25 October 2023

Journal title: available for those who has paid.

Region of the journal publication: Europe.

Scientific field: Medicine.

Indexing of the journal: Scopus (Q2); WoS (Science Citation Index Expanded) IF 3.5.

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#39-med Effect assessment of monocyte subpopulations on the immunopotentiating and tolerogenic properties of dendritic cells generated by them

Abstract: Dendritic cells perform important immunoregulatory functions while being easily generated from a population of peripheral blood monocytes. The lack of research in the field of monocyte effect in the context of monocyte subpopulations on the properties of the dendritic cells generated by them determines the relevance of the research. The purpose of this [...] Load More paper is to assess the effect of monocyte subpopulations on the immunopotentiating and tolerogenic properties of dendritic cells. The basis of the study was the data concerning the functional activity indices of dendritic cells generated by CD16- and CD16+ (CD16-Mo-DK and CD16+Mo-DK, respectively) monocytes of 39 healthy donors. The dendritic cell reaction was studied under standard conditions and experimental conditions by adding dexamethasone to dendritic cell culture. The Wilcoxon Rank-Sum Test and correlation analysis in Statistica 6.0 software package have been used for statistical data processing. The statistical significance of differences in the level of immunopotentiating and tolerogenic properties of dendritic cells has been assessed using the Wilcoxon Rank-Sum Test. The strength of the relationship between the stimulant activity of T cells and the pro-inflammatory and anti-inflammatory cytokine production has been assessed by the Spearman's rank correlation. According to the results of the assessment for standard conditions, it was found that for the CD16+Mo-DK population the endocytosis has a lower level compared with CD16-Mo-DK but is more elastic under the effect of dexamethasone. The assessment of the proliferative response has demonstrated that dendritic cells have a stimulating effect on the proliferation of T cells. At the same time, CD16+Mo-DK has a higher ability to stimulate the proliferation of allogeneic T cells. Differences in the response level of autologous T cells are not statistically significant. The dendritic cells under study are equally capable of producing pro-inflammatory and anti-inflammatory cytokine. Based on the results of the experiment, the character of the dexamethasone effect on the investigated characteristics of immunopotentiating and tolerogenic properties of dendritic cells was determined, which is stimulating for the ability of endocytosis and production of pro-inflammatory cytokine and, at the same time, discouraging for the power of proliferative response and production of pro-inflammatory cytokine. The results of the correlation analysis made it possible to determine the feedback between the stimulant activity of T cells and the production of cytokine. The exception is the direct relationship between the activity of CD16-Mo-DK and the production of the pro-inflammatory cytokine. The findings of the research have been confirmed by statistical indicators and present the basis for using regularities of monocytes' effect on the properties of dendritic cells generated by them in practical immunology.
Keywords: monocytes, dendritic cells, T cells, endocytosis, proliferation, population.
Date of publication: March 2024 / Deadline: 25 October 2023

Journal title: available for those who has paid.

Region of the journal publication: Europe.

Scientific field: Medicine.

Indexing of the journal: Scopus (Q2); WoS (Science Citation Index Expanded) IF 3.5.

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#30-med The assessment model of gene expression of early and delayed responses in the process of adaptation of muscle cells to aerobic physical activity

Abstract: Physical activity leads to changes in gene expression, which promotes the development of metabolic activity and functional capacities of skeletal muscles, improving the functional state of the body. Despite the awareness of the importance of physical activity in the development of the organism, there is no complete analytical description of the impact [...] Load More of physical activity on the processes of genetic regulation in muscle cells in the scientific literature. The purpose of this paper was to build a model and determine parameters influencing gene expression of early and delayed responses in the process of adaptation of muscle cells to physical activity. As a result of the research, a model of Ca2+-dependent signaling pathway has been built. This pathway leads to gene expression of early and delayed responses in the process of adaptation of muscle cells to physical activity. The model represents a system of ordinary differential equations, for the construction of which mechanisms of mono- and bimolecular reactions and generalized Hill's functions have been used. Sensitivity analysis has been performed to assess the effect of changes in model parameters on changes in mRNA gene concentrations. For the modeling, the BioUML software package was used with the help of which the system of differential equations has been solved (by VODE method), and the sensitivity of the developed model to changes in the rates of Ca2+-dependent signal transmission reactions and transcription regulation processes (local search algorithm) has been assessed. The model was tested using the RNA-sequencing method based on the evaluation of changes in gene expression between working and non-working skeletal muscle of seven men participating in the experiment, the matter of which was to measure the transcriptomic response in the human muscle to aerobic physical activities. The results of the simulation have demonstrated that the mRNA concentration of early response genes depends on the parameters that characterize the reaction of activation of Ca2+-dependent signaling pathway and the processes of genetic regulation, which is confirmed by the highest sensitivity indicators. The concentration of mRNA of delayed response genes is sensitive to the constitutive rate of expression and the rate of mRNA degradation. The built model serves as a diagnostic tool for taking into account the activating mechanism of aerobic physical activity impact on muscle cell gene expression.
Keywords: expression, genes, aerobic physical activities, muscle cells, adaptation, transcriptional response.
Date of publication: March 2024 / Deadline: 25 October 2023

Journal title: available for those who has paid.

Region of the journal publication: Europe.

Scientific field: Medicine.

Indexing of the journal: Scopus (Q2); WoS (Science Citation Index Expanded) IF 3.5.

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#28-med Improvement of differential diagnostics of sarcomatoid renal cell carcinoma

Abstract: Sarcomatoid carcinoma is a malignant tumor that grows from the renal tubular epithelial cells. Today, sarcomatoid renal cell cancer ranks first in the mortality rate of patients and third in the prevalence rate among all urological tumors. It occupies the leading position by the level of growth among onco-urological diseases. The survival rate is directly [...] Load More proportional to the presence of metastases in human lymph nodes, which necessitates early and effective diagnosis of the disease. Therefore, clinical diagnostics of space-occupying lesion of kidneys at an early stage is still an urgent task. The purpose of this study was to evaluate the predictive value of expression of a group of proteins associated with tumor progression, depending on the sarcomatoid differentiation of renal cell carcinoma. The clinical data for the study were the archive panel of the incisional biopsy material of 49 patients of the average age category of 67 years formed between 2011 and 2019. Based on a histopathological examination, the degree of malignancy of sarcomatoid transformation in chromophobe renal cell carcinoma, according to ISUP classification 2018, has been assessed and analyzed. The assessment has been performed by determining the percentage ratio of sarcomatoid cells to the total number of mutant pathological cells. With the use of the international TNM system, four neoplasm classes have been formed depending on the level of sarcomatous component and intervals of their variation. Four modifications of antigen expression in carcinomatous and sarcomatoid components have been substantiated for immunohistochemical reaction research and analysis. According to the results of immunohistochemical research of spindle-cell mesenchymal kidney tumors, the expression of specific markers of renal cell carcinoma in various histological modifications of sarcomatoid renal cell carcinoma has been systemized and analyzed. Distinguishing characters of expression for each marker in carcinoma and sarcomatoid components depending on the histological variant of sarcomatoid carcinoma have been determined by statistical data processing using electronic tables in Statistica10 software package based on the calculation of the Pearson's chi-square test and Fischer’s exact test (χ2). The results obtained will be useful for the development of algorithms to diagnose sarcomatoid carcinoma, which will improve the effectiveness of the research of urological tumors depending on their histological type.
Keywords: immunohistochemical research, sarcomatoid carcinoma, diagnostics, kidneys, tumor, expression, proteins.
Date of publication: March 2024 / Deadline: 25 October 2023

Journal title: available for those who has paid.

Region of the journal publication: Europe.

Scientific field: Medicine.

Indexing of the journal: Scopus (Q2); WoS (Science Citation Index Expanded) IF 3.5.

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